Application of laccases, produced by ganoderma species, for the detoxification of some aniline and phenol derivatives

Abstract

The objective of this work was to collect isolates of the white rot fungus Ganoderma from decaying woods
and investigate their laccase producing and detoxification abilities against aniline and phenol compounds.
Sporocarps were collected and the tissue culturing technique was used for fungal isolation. In these
experiments specific medium, containing benomyl, dichloran and guaiacol was used. Laccase producing of
the isolates were tested in liquid media containing different inducers. Crude enzyme extracts were prepared
and characterized. The best laccase-producing strains were identified with partial ITS sequence analysis.
Their secreted laccases were investigated with SDS-PAGE and the molecular weights of these enzymes were
estimated. Degradation of 7 different aniline and phenol derivates (2,4-dichlorophenol, 2-methyl-4-
chlorophenol, 3-chloroaniline, 4-chloroaniline, 2,6-dimethylaniline, 3,4-dichloroaniline and 3-chloro-4-
methylaniline) were investigated, observing high degradation rates in most cases.